3 tips to choose the consumables for cell culture
1. Determine cultivation mode
According to different growth modes, cells can be divided into adherent cells and suspended cells, and there are also cells that can grow adherent or suspended, such as SF9 cells. Different cells also have different requirements for cell culture consumables. Adherent cells generally use TC-treated consumables, while suspended cells do not have such requirements, but TC-treated consumables are also suitable for the growth of suspended cells. To select suitable consumables, first of all, determine the cell culture mode according to the cell type.
2. Select consumable type
Common cell culture consumables include cell culture plate, culture bottle, culture dish, triangular shake flask, pipette, etc. These consumables have different characteristics in culture area, use method, overall structure, etc. The culture bottle belongs to closed culture, which can reduce pollution; The culture plate and culture dish belong to semi-open culture, which is convenient for control experiment and gradient experiment, but it is also easier to bring bacterial pollution, which requires higher requirements for operators.
In short, when selecting the type of consumables, we should take into account the experimental needs and personal operating preferences.
3. Selected consumable specifications
Large-scale cell culture experiments need consumables with larger culture area as support, while small-scale experiments choose consumables with smaller area. Cell factories are mostly used for large-scale cell culture, such as vaccine production, monoclonal antibody, pharmaceutical industry, etc; Culture plate, culture dish and culture bottle are suitable for small-scale cell culture in the laboratory; In addition to suspension cell culture, the triangular flask can also be used for the preparation, mixing and storage of culture medium. Determine the specific specifications of consumables according to the scale of cell culture.
Proper cell culture consumables are the premise to ensure the good growth of cells, and also the key to accelerate the experimental process and ensure the culture effect. The selection should take into account the factors such as cell culture mode, culture scale and laboratory conditions.
Of course, it is also important to choose a platform with diversified products, stable supply of goods, guaranteed quality and services, such as Labio, Labio can provide comprehensive one-stop procurement services for scientific research supplies for laboratories in the fields of global life science, pharmaceutical industry, environmental protection, food safety, government agencies and clinical medicine.
Post time: Feb-09-2023