Differences between sealed cap and breathable cap of cell culture bottle

   The environment for cell culture includes sterility, appropriate temperature (37~38 ℃), osmotic pressure (260~320mmol/L), carbon dioxide and appropriate PH (7.2~7.4). Cell culture square bottles generally need to use the incubator or greenhouse for cell culture. According to the different use environment, their covers are divided into two types: sealed cover and breathable cover.

  Breathable cover: the cover is provided with air holes, which can allow carbon dioxide in the environment to enter the cell culture bottle, creating suitable growth conditions for cell growth. There is a layer of sterile breathable film on the top of the bottle cap, which has good waterproof and breathable functions. The liquid in the cell culture bottle will not affect the microbial barrier and breathable effect of the breathable film after contacting, which can ensure the good growth of cells.

   The two caps of the cell culture square bottle meet the needs of different culture environments for cell growth. When selecting the cell culture square bottle, choose the appropriate cap according to the specific environment of cell culture to avoid affecting cell growth.