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Instructions for use, cleaning, classification and use of cell culture dishes (1)

1. Instructions for use of cell culture dishes


Petri dishes are generally made of glass or plastic, and are commonly used as experimental consumables for cultivating microorganisms or cell cultures. Generally, glass dishes can be used for plant materials, microbial cultures, and adherent cultures of animal cells. The plastic material may be polyethylene material, which is suitable for laboratory inoculation, scribing, and bacteria separation operations, and can be used for the cultivation of plant materials. Petri dishes are fragile, so they should be handled with care during cleaning and use. After use, they should be cleaned in time and stored in a safe and fixed location.

 

2. Cleaning of Petri dishes

1.) Soak: Soak new or used glassware with clean water to soften and dissolve the attachment. Before using new glassware, simply brush it with tap water, and then soak it in 5% hydrochloric acid overnight; The used glassware often contains a lot of protein and oil, which is not easy to brush off after drying, so it should be immersed in clean water immediately after use for brushing.
2.) Brushing: put the soaked glassware into detergent water, and brush repeatedly with a soft brush. Do not leave dead corners and prevent damage to the surface finish of containers. Wash and dry the cleaned glassware for pickling.
3.) Pickling: Pickling is to soak the above vessels into the cleaning solution, also known as acid solution, to remove the possible residues on the surface of vessels through the strong oxidation of acid solution. Pickling shall not be less than six hours, generally overnight or longer. Be careful when placing and taking utensils.
4.) Rinsing: The vessels after brushing and pickling must be fully rinsed with water. Whether the vessels are washed clean after pickling directly affects the success of cell culture. For manual washing of pickled vessels, each vessel shall be repeatedly “filled with water – emptied” for at least 15 times, and finally soaked with re distilled water for 2-3 times, dried or dried, and packed for standby.

 


Post time: Sep-19-2022