single-header-banner

Instructions for use, cleaning, classification and use of cell culture dishes (2)

Classification of Petri dishes——

 

1. According to the different uses of culture dishes, they can be divided into cell culture dishes and bacterial culture dishes.

 

2. It can be divided into plastic petri dishes and glass petri dishes according to different manufacturing materials, but most of the imported petri dishes and disposable petri dishes are plastic materials.

 

3. According to different sizes, they can be generally divided into 35mm, 60mm and 90mm in diameter. 150mm Petri dish.

 

4. According to the difference of separation, it can be divided into 2 separate Petri dishes, 3 separate Petri dishes, etc.

 

5. The materials of culture dishes are basically divided into two categories, mainly plastic and glass. The glass can be used for plant materials, microbial culture, and adherent culture of animal cells. Plastic materials may be polyethylene materials, which can be used once or for many times. They are suitable for laboratory inoculation, scribing, and bacteria separation operations, and can be used for the cultivation of plant materials.

 

Why is the Petri dish upside down in lithographic culture——
1. During operation, there may be water drops or bacteria on the cover of the petri dish. The upside down culture can prevent the water drops or microorganisms on the cover from falling on the petri dish.
2. During the culture process, the bacteria will produce some metabolites harmful to the growth and reproduction of the bacteria during the metabolic reproduction process, release heat and discharge water. If the bacteria are not cultured upside down, water drops will fall into the culture medium, affecting the growth of the colonies.
3. If the objective of culture is to collect bacterial metabolites, and the metabolites are easily soluble in water, inverted culture may facilitate collection.
During the culture, there will be more water vapor in the culture dish, and the condensation of water vapor on the dish cover will produce water droplets. If the culture dish is placed in the right position, the water droplets will disperse the colonies. In this way, a large colony may disperse into many small colonies, causing great trouble for the cultivation and counting of bacteria. If it is caused, the culture medium is on the top and the dish is under the cover, and water drops will not drop onto the colony.
Precautions for use of Petri dishes——
1. After cleaning and disinfection before use, the cleanliness of culture dishes has a great impact on the work, which can affect the pH of the culture medium. If there are some chemicals, they will inhibit the growth of bacteria.
2. The newly purchased culture dishes should be washed with hot water first, and then immersed in 1% or 2% hydrochloric acid solution for several hours to remove free alkaline substances, and then washed twice with distilled water.
3. To cultivate bacteria, use high pressure steam (generally 6.8 * 10 Pa high pressure steam to the 5th power), sterilize it for 30min at 120 ℃, dry it at room temperature, or use dry heat to sterilize, that is, put the culture dish in the oven, control the temperature at 120 ℃ for 2h, and then kill the bacterial teeth.
4. Only sterilized culture dishes can be used for inoculation and cultivation.

Why is the Petri dish upside down in lithographic culture——
1. During operation, there may be water drops or bacteria on the cover of the petri dish. The upside down culture can prevent the water drops or microorganisms on the cover from falling on the petri dish.
2. During the culture process, the bacteria will produce some metabolites harmful to the growth and reproduction of the bacteria during the metabolic reproduction process, release heat and discharge water. If the bacteria are not cultured upside down, water drops will fall into the culture medium, affecting the growth of the colonies.
3. If the objective of culture is to collect bacterial metabolites, and the metabolites are easily soluble in water, inverted culture may facilitate collection.
During the culture, there will be more water vapor in the culture dish, and the condensation of water vapor on the dish cover will produce water droplets. If the culture dish is placed in the right position, the water droplets will disperse the colonies. In this way, a large colony may disperse into many small colonies, causing great trouble for the cultivation and counting of bacteria. If it is caused, the culture medium is on the top and the dish is under the cover, and water drops will not drop onto the colony.

 


Post time: Sep-20-2022