The differences between ELISA Plate, Cell Culture Plate, PCR Plate, and Deep Well Plate

The differences between ELISA Plate, Cell Culture Plate, PCR Plate, and Deep Well Plate

1. ELISA Plate

ELISA plate is Generally made of polystyrene, is a kind of equipment used in conjunction with a microplate reader for enzyme-linked immunoassay experiments. In ELISA, antigens, antibodies and other biomolecules are adsorbed to the surface of the microplate through various mechanisms, and then react with the tested sample and the enzyme-labeled antigen or antibody in different steps, and are detected by a microplate reader .

2. Cell Culture Plate

Cell culture plates are used to grow cells or bacteria. There are 6 holes, 12 holes, 24 holes, 48 ​​holes and 96 holes. It looks similar to a transparent microtiter plate, but its use is very different. Add an appropriate amount of culture medium to the wells of the culture plate, and then culture the cells in a suitable environment. General culture plates are flat-bottomed, suitable for suspension culture of cells and tissues, and there are also U-shaped bottoms and V-shaped bottoms. After surface modification treatment, it can make it have cell adherent culture and growth performance. Material is polystyrene.

Cell culture plates are mainly used for cell culture and can also be used to measure protein concentration; when testing the absorbance of visible light wavelengths, most 96-well transparent plates can be used. However, when testing high-precision and special wavelengths, in order to avoid the influence caused by the absorption of the orifice plate, a special microplate should be used.

3. PCR Plate

The PCR plate is used in the PCR instrument, which is the same as the use of the microplate plate with the microplate reader. It is used as a solid phase carrier, allowing the sample to undergo PCR reaction in it, and then use the PCR instrument for detection. In fact, to put it simply, a PCR plate is a combination of many PCR tubes, usually 96 wells. Generally made of PP material.

4. Deep Well Plate

Microplates such as microplates and PCR plates can be used as microwell plates because the volume of each well is very small. There is also a plate in the laboratory with relatively deep holes called Deep Well Plates. It is made of polymer PP material and has good chemical compatibility, can be used for storage of most polar organic solutions, acidic and alkaline solutions and other laboratory liquids.