Usage and precautions of pipette
1. Installation of pipette tips
For the single channel pipette, the end of the pipette is vertically inserted into the suction head, and it can be tightened by gently pressing it left and right slightly;
For multi-channel pipettes, align the first pipette with the first suction head, insert it obliquely, shake it back and forth slightly and tighten it.
Do not repeatedly hit the pipette to ensure the air tightness of the suction head. If the suction head is assembled in this way for a long time, the parts of the pipette will become loose due to strong impact, or even the knob for adjusting the scale will get stuck.
2. Capacity setting
When adjusting from large volume to small volume, rotate it counterclockwise to the scale; When adjusting from small volume to large volume, you can adjust the set volume clockwise first, and then back to the set volume to ensure the best accuracy.
Do not turn the adjusting knob out of the range, or the mechanical device in the pipette will be damaged.
3. Suction and discharge
Press the liquid aspirating pipette button to the first gear and release the button to aspirate. Be sure not to go too fast, otherwise the liquid will enter the suction head too fast, which will cause the liquid to be sucked back into the pipette.
The liquid drain is close to the container wall. Press it to the first gear, pause slightly, and then press it to the second gear to drain the residual liquid.
● Suck liquid vertically.
● For 5ml and 10ml pipettes, the suction head needs to immerse into the liquid level for 5mm, slowly suck the liquid, after reaching the predetermined volume, pause under the liquid level for 3s, and then leave the liquid level.
● Loosen the controller slowly when aspirating, otherwise the liquid will enter the suction head too quickly, which will cause the liquid to be sucked back into the pipette
● When absorbing volatile liquid, wet the suction head 4-6 times to saturate the steam in the sleeve chamber to avoid liquid leakage.
4. Correct placement of pipette
After use, it can be hung vertically on the liquid transfer gun rack, but be careful not to fall off. When there is liquid in the gun head of the pipette, do not place the pipette horizontally or upside down to avoid the reverse flow of liquid corroding the piston spring.
If it is not used, adjust the measuring range of the liquid transfer gun to the maximum scale, so that the spring is in a relaxed state to protect the spring.
5. Common error operations
1) When assembling the suction head, the suction head is repeatedly impacted, which makes it difficult to unload the suction head, or even damages the pipette.
2) When aspirating, the pipette inclines, resulting in inaccurate liquid transfer, and the liquid is easy to enter the handle of the pipette.
3) When sucking, the thumb is released quickly, which will force the liquid to form a turbulent state, and the liquid will directly rush to the inside of the pipette.
4) Press it directly to the second gear for aspirating (the above standard method should be followed).
5) Use a large range pipette to transfer a small volume of sample (a pipette with a suitable range should be selected).
6) Place the pipette with residual liquid suction head horizontally (the pipette shall be hung on the pipette rack).
Post time: Nov-30-2022