Cell culture refers to a method that simulates the internal environment (sterility, appropriate temperature, pH and certain nutritional conditions, etc.) in vitro to make it survive, grow, reproduce and maintain its main structure and function. Cell culture is also called cell cloning technology. In biology, the formal term is cell culture technology. Whether for the whole bioengineering technology or one of the biological cloning technologies, cell culture is an essential process. Cell culture itself is the large-scale cloning of cells. Cell culture technology can turn a cell into a simple single cell or a few differentiated multi cells through mass culture, which is an essential link of cloning technology, and cell culture itself is cell cloning. Cell culture technology is an important and commonly used technology in cell biology research methods. Cell culture can not only obtain a large number of cells, but also study cell signal transduction, cell anabolism, cell growth and proliferation.