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Liquid volume and shaking speed of triangular shake flask for cell culture applications

Animal/plant cell culture technology is a series of biotechnology operations performed on isolated plant cells or protoplasts at the cellular level for a certain purpose. It includes isolation, culture, regeneration and a series of related operations. As far as the production of useful compounds is concerned, it mainly refers to the process of producing useful compounds through suspension culture of plant cells under sterile conditions.

Shandong Labio sterile cell triangular shake flask is specially designed for the culture of suspension cells 293, CHO and other cells. The shake flask has a flat bottom and a breathable cover. It can be used in small-scale process development, step-by-step amplification and other culture stages. The breathable cover has a 0.2μm breathable film, which is breathable and impermeable to water, which can effectively prevent the entry of microorganisms, prevent contamination, and ensure gas exchange, allowing cells to grow well.

When using a triangular shake flask, it is best to control the amount of culture medium added to 20 to 30% of the volume of the flask. There are clear graduation lines on the triangular shake flask for easy reference. The rotation speed of the shaker is recommended to be controlled at 75~125RPM, which can be adjusted according to the actual situation.


Post time: Dec-21-2023