Sample collection, storage and transportation requirements for common experiments

Sample collection, storage and transportation requirements for common experiments

1. Collection and preservation of pathological specimens:

☛Frozen section: Remove appropriate tissue blocks and store them in liquid nitrogen;

☛Paraffin sectioning: Remove appropriate tissue blocks and store them in 4% paraformaldehyde;

☛Cell slides: Cell slides were fixed in 4% paraformaldehyde for 30 minutes, then replaced with PBS and immersed in PBS and stored at 4°C.

2. Collection and preservation of molecular biology specimens:

☛Fresh tissue: Cut the specimen and store it in liquid nitrogen or -80°C refrigerator;

☛Paraffin specimens: Store at room temperature;

☛Whole blood specimen: Take an appropriate amount of whole blood and add EDTA or heparin anticoagulation blood collection tube;

☛Body fluid samples: high-speed centrifugation to collect the sediment;

☛Cell specimens: Cells are lysed with TRizol and stored in liquid nitrogen or -80°C refrigerator.

3. Collection and storage of protein experiment specimens:

☛Fresh tissue: Cut the specimen and store it in liquid nitrogen or -80°C refrigerator;

☛Whole blood specimen: Take an appropriate amount of whole blood and add EDTA or heparin anticoagulation blood collection tube;

☛Cell specimens: Cells are fully lysed with cell lysis solution and then stored in liquid nitrogen or -80°C refrigerator.

4. Collection and storage of ELISA, radioimmunoassay, and biochemical experiment specimens:

☛Serum (plasma) sample: Take whole blood and add it to a procoagulation tube (anticoagulation tube), centrifuge at 2500 rpm for about 20 minutes, collect the supernatant, and store it in liquid nitrogen or in a -80°C refrigerator;

☛Urine sample: centrifuge the sample at 2500 rpm for about 20 minutes, and store it in liquid nitrogen or -80°C refrigerator; refer to this method for thoracic and ascites fluid, cerebrospinal fluid, and alveolar lavage fluid;

☛Cell samples: When detecting secreted components, centrifuge the samples at 2500 rpm for about 20 minutes and store them in liquid nitrogen or -80°C refrigerator; when detecting intracellular components, Dilute the cell suspension with PBS and freeze and thaw repeatedly to destroy the cells and release intracellular components. Centrifuge at 2500 rpm for about 20 minutes and collect the supernatant as above;

☛Tissue samples: After cutting the specimens, weigh them and freeze them in liquid nitrogen or -80°C refrigerator for later use.

5. Metabolomics specimen collection:

☛Urine sample: Centrifuge the sample at 2500 rpm for about 20 minutes and store it in liquid nitrogen or -80°C refrigerator; refer to this method for thoracic and ascites fluid, cerebrospinal fluid, alveolar lavage fluid, etc.;

☛After cutting the tissue sample, weigh it and freeze it in liquid nitrogen or a -80°C refrigerator for later use;