The ELISA plate is an indispensable tool for ELISA, the enzyme-linked immunosorbent assay. There are many factors that affect the success of ELISA experiments. Choosing the right tool is the first step. Choosing a suitable microplate will help the experiment to be successful.
The material of the ELISA plate is generally polystyrene (PS), and polystyrene has poor chemical stability and can be dissolved by a variety of organic solvents (such as aromatic hydrocarbons, halogenated hydrocarbons, etc.), and can be corroded by strong acids and alkalis. Not resistant to grease and easily discolored after being exposed to UV light.
What types of ELISA plates are there?
✦Select by color
Transparent plate: suitable for quantitative and qualitative solid-phase immunoassays and binding assays;
White plate: suitable for self-luminescence and chemiluminescence;
Black plate: suitable for fluorescent immunoassays and binding assays.
✦Select by binding strength
Low-binding plate: Passively binds to proteins through surface hydrophobic bonds. It is suitable as a solid-phase carrier for macromolecular proteins with a molecular weight >20kD. Its protein-binding capacity is 200~300ng IgG/cm2.
High binding plate: After surface treatment, its protein binding capacity is greatly enhanced, reaching 300~400ng IgG/cm2, and the molecular weight of the main bound protein is >10kD.
✦Sort by bottom shape
Flat bottom: low refractive index, suitable for detection with microplate readers;
U bottom: The refractive index is high, which is convenient for adding, aspirating, mixing and other operations. You can directly observe the color changes by visual inspection without placing it on the microplate reader to determine whether there is a corresponding immune reaction.
Post time: Dec-22-2023