Cell culture is a matter of stabbing the heart and the lungs. You should treat it as carefully as a child, love her and take care of her. If you pay attention to these problems when taking care of them, your cells will be better nourished. Now let’s talk about the precautions of cell culture.
Preparation before cell culture
Before you wear gloves to start cell culture, check whether the number of pipettes and bottles is sufficient, so as to avoid entering and leaving the console again after the experiment, which can reduce the risk of cell pollution.
The cell culture medium should also be preheated first. Choosing to preheat only part of the medium rather than the whole bottle can not only save experimental time, but also avoid protein degradation caused by repeatedly heating the medium.
After the operation, do not forget that the medium is sensitive to light and should be kept away from light as much as possible.
Periodic inspection of cell culture
Regular examination of the morphology of cultured cells, that is, shape and appearance, is essential for the success of cell culture experiments.
In addition to confirming the healthy state of cells, examining cells with naked eyes and microscope each time you operate cells can also find signs of pollution early, so as to avoid the spread of pollution to other cells in the laboratory.
Signs of cell degeneration
The signs of cell degeneration include the appearance of granules around the nucleus, the dissociation of cells from the matrix, and the formation of cytoplasmic vacuoles.
These metamorphic signs may be caused by many reasons, such as:
Contamination of the culture, cell line senescence, or presence of toxic substances in the culture medium, or these signs only indicate that the culture needs to be replaced.
When the metamorphism is serious, it will become irreversible change.
Disinfection and layout of cell culture fume hood
Keep the cell culture fume hood clean and orderly, and place all objects within the direct view range.
Spray 70% ethanol on all articles put into the fume hood, wipe and clean them for disinfection.
Place a cell culture container in the open place in the middle of the fume hood; The pipette is placed at the right front for easy access; The reagent and culture medium are placed at the right back for easy absorption; The test tube rack is arranged at the middle rear part; A small container is placed at the left rear to hold waste liquid.
Post time: Nov-18-2022